An aptasensor for electrochemical detection of tumor necrosis factor in human blood.

نویسندگان

  • Ying Liu
  • Qing Zhou
  • Alexander Revzin
چکیده

Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α) - a key inflammatory cytokine - in whole human blood. When testing spiked blood, a TNF-α detection limit of 58 pM (10 ng mL(-1)) and a linear range of 6 nM (100 ng mL(-1)) could be achieved. Furthermore, detection of TNF-α in mitogen stimulated whole blood was demonstrated.

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عنوان ژورنال:
  • The Analyst

دوره 138 15  شماره 

صفحات  -

تاریخ انتشار 2013